DLS relies on the principle that freely diffusing material, moving randomly due to Brownian motion, will produce rapid fluctuations in scattered laser light. The timescale of these intensity fluctuations is on the order of tens of nanoseconds to hundreds of milliseconds. These fluctuations are directly related to the motion of particles. The signal that arises from the scattered intensity from the laser light is acquired and transformed into an autocorrelation function. This is the basis for measuring a particle size distribution.
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