Applications and Articles - Testa Analytical Solutions

CHROMATOGRAPHY

1% Abweichung, 40% Fehler: So htig ist Durchflussmessung

Kalibrierungen sind Pflicht für wiederholgenaue Ergebnisse. Doch gerade aufwendige Messreihen wie in der Gel-Permeations-Chromatographie dauern oft lange, sodass die tatsächliche Flussrate minimal abweichen kann. Welch enorme Folgen dies nach sich zieht, zeigt das folgende Beispiel.
Published in LaborPraxis – April 2025
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Effects of small deviations in flow rate on GPC/SEC results

GPC/SEC is undoubtedly one of the most powerful techniques for the characterisation of natural and synthetic polymers. Its fexibility and the high information content available from just a single measurement, makes it the technique of choice for simple determination of molecular weight distribution and polydispersity of the most complex structure analysis or co-polymer composition distributions.
Published in Lab Asia – April 2025
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El refractómetro differencial mejora la determinación del peso molecular por dispersión de la luz

El refractómetro differencial de Testa Analytical mejora la precisión de la determinación de dn/dc utilizando técnicas de dispersión de luz.
Published in Tecnicas de Laboratorio – April 2025
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HPLC qualification for GMP-regulated labs

Qualification of HPLC Systems is one of the most common tasks in GMP-regulated laboratories. Several different regulatory guidelines and pharmacopeial standards offer frameworks for this, ranging from pure instructions to rigid quality standards to be checked and documented in regular intervals.
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Effects of small deviations in flowrate on GPC/SEC results

GPC/SEC is undoubtedly one of the most powerful techniques for the characterisation of natural and synthetic polymers. Its fexibility and the high information content available from just a single measurement, makes it the technique of choice for simple determination of molecular weight distribution and polydispersity to the most complex structure analysis or co-polymer composition distribution.
Published in International Labmate – March 2025
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Comparing volumetric and thermal flowmeters for assessing and validating liquid chromatography performance

Assessing and validating the performance of a pump serving HPLC, UHPLC, Ion Chromatography of GPC/SEC system is a crucial task in many laboratories these days.
Published in International Labmate – February 2025
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Comparing volumetric and thermal flowmeters for assessing and validating liquid chromatography performance

Assessing and validating the performance of a pump serving HPLC, UHPLC, Ion Chromatography of GPC/SEC system is a crucial task in many laboratories these days.
Published in Lab Asia – February 2025
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Comparaison des débitmètres volumétriques et thermiques pour l'évaluation et la validation des performances de la chromatographie liquide

L’évaluation et la validation des performances d’une pompe destinée aux systèmes HPLC, UHPLC, de chromatographie ionique ou de GPC/SEC est une tâche cruciale dans de nombreux laboratoires.
Published in La Gazette du Laboratoire – December 2024
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Comparación de caudalímetros volumétricos y térmicos para evaluary y validar el rendimiento de la cromatografía líquida

Evaluar y validar el rendimiento de una bomba al servicio de un sistema de HPLC, UHPLC, cromatografía iónica o GPC/SEC es una tarea crucial en muchos laboratorios hoy en día. Para ello, la determinación precisa del caudal es una herramienta poderosa.
Published in Tecnicas de Laboratorio – December 2024
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Testa Analytical's monitoring device used to validate preparative HPLC pump

Regular pump flow rate validation is crucial to ensuring pump performance, repeatability, and compliance with regulations. In this investigation, a non-invasive, real time flow monitoring device – the Prep Flowmeter was used to validate a Teledyne SSI M1 40ml single-headed, positive displacement piston pump commonly used for preparative liquid chromatography applications.
Published in Scientist Live – December 2024
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Validation du gradient HPLC à l'aide de débitmètres non invasifs

La CLHP à gradient est un outil essentiel pour l’analyse d’échantillons contenant des composés de polarité variable. Son efficacité découle de la possibilité d’adapter à la fois la durée et la composition du gradient, ce qui permet de manipuler les temps de rétention des analytes.
Published in La Gazette du Laboratoire – October 2024
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A time-saving pump validation method for preparative chromatography

Regular pump flow rate validation is a crucial tool in ensuring pump performance, repeatability, and compliance with regulations. In this investigation, a non-invasive, real time flow monitoring device – the Prep Flowmeter was used to validate a Teledyne SSI M1 40ml single-headed, positive displacement piston pump commonly used for preparative liquid chromatography applications.
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HPLC gradient validation using non-invasive flowmeters

Gradient HPLC serves as a crucial tool for analysing samples containing compounds with varying polarity. Its effectiveness stems from the ability to tailor both gradient time and composition, enabling the manipulation of analyte retention times. However, challenges arise from deviations in the programmed gradient profiles produced by HPLC systems, impacting retention time and peak shape. Several factors contribute to this variability in gradient HPLC, such as solvent quality, pump performance, changes in system pressure, and the condition of the columns. Addressing these challenges requires precise control and monitoring of gradient profiles.
Published in International Labmate – September 2024
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Validación de gradientes de HPLC con caudalímetros no invasivos

La HPLC de gradiente es una herramienta crucial para analizar muestras que contienen compuestos de polaridad variable. Su eficacia radica en la capacidad de adaptar tanto el tiempo como la composición del gradiente, lo que permite manipular los tiempos de retención de los analitos.
Published in Tecnicas de Laboratorio – July/August 2024
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Enhancing HPLC Field Service with fast-response, non-invasive flowmeters

Field Service Engineers are crucial to scientists striving to get the most from their analytical equipment. The maintenance, performance assurance and validation of HPLC/uHPLC systems gains particular importance when this equipment is an essential component in labs subject to compliance found in the pharmaceutical, food, beverage, and environmental analysis sectors.
Published in Lab Asia – June 2024
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Validating HPLC results obtained using any solvent

High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, facilitating the separation, identification, and quantification of chemical and biological compounds. While many applications are based upon aqueous eluents, HPLC separation protocols also often involve the utilization of other solvents to accommodate the unique properties of different analytes. Ensuring consistent elution profiles is a key requirement to ensure reproducible results. Continuous monitoring of the delivered HPLC eluent flow rate is therefore essential when permanent validation of results is desired or necessary as it is in regulated industries.
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